| D.R.E. ENVIRONMENTAL INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Sep 13, 1990 | Inactive |
| SUWANNEE AMUSEMENT COMPANY, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Jan 25, 1988 | Inactive |
| SUWANNEE VALLEY SYSTEMS, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Feb 19, 1988 | Inactive |
| FISH FRY CLUB, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Mar 1, 1988 | Inactive |
| SHREEJEE INVESTMENTS, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Jun 10, 1988 | Inactive |
| TIMELY MATERIAL ASSOCIATES, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Jul 21, 1988 | Inactive |
| DAMPIERVILLE OWNERS' ASSOCIATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Oct 11, 2000 | Inactive |
| SUWANNEE VALLEY/WINFIELD VOLUNTEER FIRE DEPARTMENT, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Nov 21, 1983 | Inactive |
| BIG SLOUGH HUNTING CLUB, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | May 25, 2001 | Inactive |
| THE SPIRITUAL OUTREACH CENTER, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Oct 11, 2001 | Inactive |
| TWELVE OAKS III ASSOCIATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Feb 20, 1984 | Inactive |
| HILLHOUSE & HILLHOUSE EMPOWERMENT FOUNDATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Aug 22, 2003 | Inactive |
| THE UNIVERSITY OF FLORIDA COLUMBIA COUNTY GATOR CLUB, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Jun 7, 1984 | Inactive |
| LAKE CITY CHEERLEADERS ASSOCIATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Jan 12, 2004 | Inactive |
| CHAPTER #20, DISABLED AMERICAN VETERANS, DEPARTMENT OF FLORIDA, INCORPORATEDLake City, FL · Inactive | Lake City, FL | Nonprofit | Sep 7, 1984 | Inactive |
| FLORIDA ASSOCIATION OF PROFESSIONAL FAMILY MEDIATORS, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Oct 16, 1984 | Inactive |
| JIM CAIN MINISTRIES, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Dec 27, 2006 | Inactive |
| SUWANNEE VALLEY PERSONNEL ASSOCIATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Nov 27, 1984 | Inactive |
| D & C PROJECTS, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Oct 25, 2012 | Inactive |
| COLUMBIA COUNTY YOUTH COUNCIL, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Nov 27, 1985 | Inactive |
| PECAN RIDGE HOMEOWNERS ASSOCIATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Apr 1, 1986 | Inactive |
| THE WRONGFULLY ACCUSED PROJECT INC.Orange Park, FL · Inactive | Orange Park, FL | Nonprofit | Aug 9, 2016 | Inactive |
| LAKE CITY ~ COLUMBIA COUNTY HISTORICAL MUSEUM, INC.Lake City, FL · Active | Lake City, FL | Nonprofit | Aug 6, 1986 | Active |
| WESTSIDE ASSEMBLY OF GOD OF LAKE CITY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Aug 6, 1986 | Inactive |
| SPIRITUAL FAITH THROUGH CHRIST MINISTRY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Aug 20, 1986 | Inactive |
| UNITED CARE SERVICE OF FLORIDA, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Oct 9, 1986 | Inactive |
| WE MUST HAVE OWNERSHIP IN OUR YOUTH NOW IS OUR TIME: WHY NOT?, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Jun 13, 2018 | Inactive |
| FLORIDA K-9 ASSOCIATION, INC.Lake City, FL · Active | Lake City, FL | Nonprofit | Oct 27, 1987 | Active |
| COUNCIL OF CONCERNED CITIZENS, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Mar 2, 1988 | Inactive |
| THE TURNING POINT OF COLUMBIA COUNTY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Mar 24, 1988 | Inactive |
| NAV SHAKTI DHAM CORPORATIONLake City, FL · Active | Lake City, FL | Nonprofit | Apr 17, 2026 | Active |
| B & S COMBS ELKS LODGE #1599, INC.Lake City, FL · Active | Lake City, FL | Nonprofit | Apr 28, 1989 | Active |
| DISABLED AMERICAN VETERANS AUXILIARY, LAKE CITY UNIT #20, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Oct 31, 1989 | Inactive |
| COLUMBIA COUNTY MEMORIAL FOUNDATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Nov 6, 1989 | Inactive |
| WESTSIDE BAPTIST CHAPEL OF LAKE CITY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Jan 31, 1990 | Inactive |
| COLUMBIA ACADEMY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Aug 28, 1991 | Inactive |
| PENTICOSTAL BELIVERS OF THE COMING OF CHRIST INC.Lake City, FL · Active | Lake City, FL | Nonprofit | Sep 23, 1991 | Active |
| QUAIL RUN OWNERS ASSOCIATION, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Dec 12, 1991 | Inactive |
| THE GATEWAY CITY INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Jan 31, 1992 | Inactive |
| COLUMBIA RIDING CLUB, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Jul 23, 1993 | Inactive |
| ROLLING HILLS PLACE IMPROVEMENT ASSOCIATION, INC.Jacksonville, FL · Inactive | Jacksonville, FL | Nonprofit | Aug 4, 1993 | Inactive |
| THE AMERICAN COLLEGE OF ENVIRONMENTAL HEALTH, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Aug 24, 1993 | Inactive |
| FIRST CHRISTIAN CHURCH OF LAKE CITY, INC.Lake City, FL · Active | Lake City, FL | Nonprofit | Oct 6, 1993 | Active |
| FOLLOWERS OF CHRIST MINISTRY CORPORATIONLake City, FL · Active | Lake City, FL | Nonprofit | Mar 1, 1994 | Active |
| SISTAHS OF COLUMBIA COUNTY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Apr 11, 1996 | Inactive |
| COMMUNITY THEATER OF LAKE CITY, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Sep 19, 1997 | Inactive |
| IGLESIA DE DIOS EBENEZER, INC.Lake City, FL · Inactive | Lake City, FL | Nonprofit | Aug 18, 1999 | Inactive |
| TLC MINISTRIES, INC.Lake City, FL · Active | Lake City, FL | Nonprofit | Sep 20, 1999 | Active |
| NEW CHINA, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Feb 29, 2000 | Inactive |
| VISION TELECOMMUNICATION INTERNATIONAL, INC.Lake City, FL · Inactive | Lake City, FL | Corporation | Mar 13, 2000 | Inactive |