| ORGANIC SUPERSOIL OF FLORIDA, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Mar 12, 1979 | Inactive |
| PAN AM TRUCK WORLD, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Mar 12, 1979 | Inactive |
| WEST COAST RECYCLING INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Mar 14, 1979 | Inactive |
| BARTON BROS. CONSTRUCTION, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Apr 12, 1979 | Inactive |
| BOSCOE'S ENTERPRISES, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jun 13, 1979 | Inactive |
| MARANATHA HOME INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jul 6, 1979 | Inactive |
| H.A.F., INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jul 1, 1979 | Inactive |
| AUBURNDALE AUTOMOTIVE LEASING, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Aug 23, 1979 | Inactive |
| CUSTOM ENCLOSURES, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Sep 27, 1979 | Inactive |
| TIP-TOP APPLIANCES, INC.Auburndale, FL · Inactive | Auburndale, FL | Corporation | Feb 8, 1980 | Inactive |
| TACO WORLD, INC.Auburndale, FL · Inactive | Auburndale, FL | Corporation | Mar 17, 1980 | Inactive |
| GLENN RISDON FARMS, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Apr 1, 1980 | Inactive |
| A. WELTON INVESTMENT FLORIDA, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Apr 11, 1980 | Inactive |
| STEWART CITRUS SERVICES, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Apr 10, 1980 | Inactive |
| CENTRAL FLORIDA AGENCY, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | May 14, 1980 | Inactive |
| LAKE ALFRED ADULT CONGREGATE LIVING FACILITY, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jun 30, 1980 | Inactive |
| INDEPENDENT TRUCKER'S, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jul 24, 1980 | Inactive |
| THE SMITH LAW GROUP, P.A.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Sep 1, 1980 | Inactive |
| NORTHEAST POLK COUNTY COMMUNITY NURSING COUNCIL, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Jun 28, 1961 | Inactive |
| LAKE ALFRED CHAMBER OF COMMERCE INCLake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Sep 10, 1963 | Inactive |
| FIRST PRESBYTERIAN CHURCH OF LAKE ALFRED, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Mar 17, 1965 | Inactive |
| FIRST UNITED METHODIST CHURCH OF LAKE ALFRED, INC.Lake Alfred, FL · Active | Lake Alfred, FL | Nonprofit | Dec 7, 1967 | Active |
| LAKE ALFRED POLICE BENEVOLENT ASSOCIATION, INC.Location not reported, FL · Inactive | Location not reported, FL | Nonprofit | Jun 18, 1968 | Inactive |
| FLORIDA ASSOCIATION OF EMERGENCY MEDICAL TECHNICIANS, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Jan 12, 1971 | Inactive |
| RIDGE VETERINARY MEDICAL SOCIETY, INCLake Alfred, FL · Active | Lake Alfred, FL | Nonprofit | Apr 11, 1972 | Active |
| LAKE ALFRED LIONS CLUB, INCLake Alfred, FL · Active | Lake Alfred, FL | Nonprofit | Jun 23, 1972 | Active |
| LAKE ALFRED FIRE DEPARTMENT BENEVOLENT ASSOCIATION, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | May 22, 1973 | Inactive |
| POLK COUNTY CRIMINAL DEFENSE LAWYERS ASSOCIATION, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Mar 29, 1974 | Inactive |
| LAKE ALFRED LITTLE LEAGUE, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Sep 29, 1975 | Inactive |
| THE AGRICULTURAL AND LABOR PROGRAM, INCORPORATEDLake Alfred, FL · Active | Lake Alfred, FL | Nonprofit | May 3, 1976 | Active |
| CHRISTIAN PILOTS ASSOCIATION-IMPERIAL POLK COUNTY WING, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Sep 13, 1978 | Inactive |
| SPIRITUAL ASSEMBLY OF BAHA'IS OF POLK COUNTY EAST, INCLake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Mar 29, 1979 | Inactive |
| FIRST ASSEMBLY OF GOD OF LAKE ALFRED, FLORIDA, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Aug 31, 1979 | Inactive |
| BETHEL MISSIONARY BAPTIST CHURCH OF LAKE ALFRED, INC.Lake Alfred, FL · Active | Lake Alfred, FL | Nonprofit | Oct 15, 1979 | Active |
| LAKE MARIANA ACRES HOMEOWNERS ASSOCIATION, INC.Lake Alfred, FL · Active | Lake Alfred, FL | Nonprofit | Dec 8, 1981 | Active |
| GROWERS FERTILIZER CO-OPERATIVELocation not reported, FL · Inactive | Location not reported, FL | Nonprofit | Jan 30, 1934 | Inactive |
| GERALD L. VANDER HEYDEN FAMILY LIMITED PARTNERSHIP #1Lake Alfred, FL · Inactive | Lake Alfred, FL | Partnership | Jan 17, 2001 | Inactive |
| GERALD L. VANDER HEYDEN FAMILY LIMITED PARTNERSHIP #2Lake Alfred, FL · Inactive | Lake Alfred, FL | Partnership | Jan 17, 2001 | Inactive |
| M-2 PROPERTIES, LTD.Lake Alfred, FL · Inactive | Lake Alfred, FL | Partnership | Dec 6, 1985 | Inactive |
| INTERNATIONAL SOCIETY OF CITRICULTURE, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Nonprofit | Feb 4, 2000 | Inactive |
| GOLD COAST AUTOS, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Sep 27, 2002 | Inactive |
| GOLD COAST AUTOS INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Mar 23, 2005 | Inactive |
| KILROY PIZZA, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Nov 25, 1980 | Inactive |
| REAL ESTATE ACQUISITION CONSULTANTS OF ORLANDO, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Oct 24, 2007 | Inactive |
| DK ACUPUNCTURE & CHIROPRACTIC CORPORATIONLake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Apr 24, 2008 | Inactive |
| GOJO HOME SOLUTIONS, INCLake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jan 16, 2013 | Inactive |
| A&A MASONRY AND WATERPROOF, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Jan 17, 2020 | Inactive |
| HUDSON CONSTRUCTION GROUP INCLake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Feb 6, 2023 | Inactive |
| CUT FLOWERS WHOLESALER, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Mar 19, 1981 | Inactive |
| RICHARD R. GARDNER & SONS, INC.Lake Alfred, FL · Inactive | Lake Alfred, FL | Corporation | Mar 26, 1981 | Inactive |