| KEVIN'S CADDIES - THE GRAND CHAPTER OF FLORIDA ORDER OF THE EASTERN STAR, INC./LAKELAND CHAPTER 26Polk City, FL · Inactive | Polk City, FL | Nonprofit | Apr 3, 2023 | Inactive |
| GLOBAL AID ALLIANCE INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Jul 17, 2023 | Inactive |
| BETTER LIFE CATS, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Sep 5, 2023 | Inactive |
| POLK CITY GRANGE, NO. 212 INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Nov 10, 1987 | Inactive |
| CENTRAL FLO, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Apr 4, 2024 | Active |
| DLB CAT RESCUE INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Apr 30, 2024 | Active |
| MOMS AND STUFF CORPORATIONPolk City, FL · Inactive | Polk City, FL | Nonprofit | May 6, 2024 | Inactive |
| RESCUE CHURCH IN THE GREEN SWAMP INCPolk City, FL · Inactive | Polk City, FL | Nonprofit | Dec 3, 2024 | Inactive |
| POLK CITY LIONS CLUB, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Jan 29, 1988 | Inactive |
| WHEN THE STRUGGLE IS REAL INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Dec 4, 2025 | Active |
| PROTECTIVE ORDER OF THE SHADOW KINGDOMS CORPPolk City, FL · Active | Polk City, FL | Nonprofit | Mar 24, 2026 | Active |
| HOSTILE FASTPITCH INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Apr 17, 2026 | Active |
| TORNO DEL ALFARERO MINISTRIES INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Aug 18, 2026 | Active |
| THE FLORIDA SPORT HORSE CLUB, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Dec 6, 1988 | Active |
| NEW TESTAMENT ENDTIME MINISTRIES, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Jan 5, 1989 | Active |
| DEER TRAILS PROPERTY OWNERS' ASSOCIATION, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Nov 24, 1989 | Inactive |
| POLK COUNTY RAILS TO TRAILS, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | May 25, 1989 | Inactive |
| FLORIDA EXCEPTIONAL EQUESTRIAN LEAGUE, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Oct 5, 1989 | Inactive |
| OLIVE BRANCH RECREATION CENTER, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Mar 19, 1990 | Inactive |
| THE GOSPEL MINISTRIES OF THE HOLY SPIRIT, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Apr 18, 1990 | Inactive |
| MOUNT OLIVE BAPTIST CHURCH OF POLK CITY, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Sep 4, 1990 | Active |
| DEER TRAILS NORTH PROPERTY OWNERS' ASSOCIATION, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Mar 28, 1991 | Inactive |
| MEADOW OAKS ACRES PROPERTY OWNERS' ASSOCIATION, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Aug 12, 1991 | Inactive |
| POLK CITY YOUTH FOOTBALL, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Oct 30, 1992 | Inactive |
| SOUTHEAST AREA MOTOR COACH ASSOCIATION, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Dec 17, 1992 | Active |
| SANDY POINTE SUBDIVISION PROPERTY OWNERS ASSOCIATION, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Jan 6, 1993 | Active |
| IMPERIAL MUSTANGS OF POLK COUNTY, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Apr 20, 1993 | Active |
| CAMP GILEAD CBM MINISTRIES OF FLORIDA, INC.Polk City, FL · Active | Polk City, FL | Nonprofit | Nov 12, 1991 | Active |
| DEER TRAILS NORTH PHASE TWO PROPERTY OWNERS' ASSOCIATION, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Nov 10, 1993 | Inactive |
| POLK COUNTY FIRE DEPARTMENT AUXILIARY STATION 410 INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Jul 8, 1994 | Inactive |
| YEARLING TRACE PROPERTY OWNERS ASSOCIATION, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Oct 24, 1994 | Inactive |
| ERNEST L. JUDY MINISTRIES INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Feb 2, 1995 | Inactive |
| IN FLIGHT MINISTRIES, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Feb 6, 1995 | Inactive |
| TRIUMPH THE BROTHERHOOD HEALING TEMPLE KINGDOM OF GOD IN CHRIST INC.Polk City, FL · Active | Polk City, FL | Nonprofit | May 30, 1995 | Active |
| VIETNAM VETERANS OF AMERICA, INC., CHAPTER #726 POLK CITY, FLORIDAPolk City, FL · Inactive | Polk City, FL | Nonprofit | Jun 12, 1995 | Inactive |
| CENTRAL FLORIDA DIAMONDBACKS, INCORPORATEDPolk City, FL · Inactive | Polk City, FL | Nonprofit | Oct 12, 1995 | Inactive |
| POLK CITY AREA CHAMBER OF COMMERCE, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Oct 26, 1995 | Inactive |
| IMAGINE THAT MINISTRIES, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Sep 22, 1997 | Inactive |
| FOSTER AND ADOPTIVE FAMILIES OF POLK COUNTY, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Nov 10, 1997 | Inactive |
| SOLID ROCK BAPTIST CHURCH OF POLK CITY INCORPORATEDPolk City, FL · Inactive | Polk City, FL | Nonprofit | Apr 16, 1998 | Inactive |
| OUTREACH REVIVAL CENTER, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Oct 22, 1998 | Inactive |
| BRIDGES ECONOMIC DEVELOPMENT, INC.Polk City, FL · Inactive | Polk City, FL | Nonprofit | Nov 30, 1998 | Inactive |
| WTFS, INC.Polk City, FL · Inactive | Polk City, FL | Corporation | Feb 25, 2000 | Inactive |
| QUALITY FIRE STOPPING, INC.Polk City, FL · Inactive | Polk City, FL | Corporation | Mar 13, 2000 | Inactive |
| ELAHI CORPORATIONPolk City, FL · Inactive | Polk City, FL | Corporation | Apr 11, 2000 | Inactive |
| DEE'S AUTO TRANSPORT, INC.Polk City, FL · Inactive | Polk City, FL | Corporation | May 15, 2000 | Inactive |
| H. G. CARTER, INC.Polk City, FL · Inactive | Polk City, FL | Corporation | Jun 5, 2000 | Inactive |
| S. MILAM ENTERPRISES INCORPORATEDPolk City, FL · Inactive | Polk City, FL | Corporation | Jun 30, 2000 | Inactive |
| PASO WORLD USA, INC.Polk City, FL · Inactive | Polk City, FL | Corporation | Aug 16, 2000 | Inactive |
| K.R. DOTSON TRUCKING, INC.Polk City, FL · Inactive | Polk City, FL | Corporation | Sep 21, 2000 | Inactive |