| NORTH CAPTIVA MARINA ASSOCIATION, INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Nov 25, 2014 | Inactive |
| PARKS FOR THE ARTS INC.Saint James City, FL · Inactive | Saint James City, FL | Nonprofit | Mar 3, 2015 | Inactive |
| NORTH CAPTIVA COMMUNITY BEACH RESTORATION INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Mar 15, 2016 | Inactive |
| PATRIOT DNA RESEARCH INCSt. James City, FL · Inactive | St. James City, FL | Nonprofit | Jul 7, 2016 | Inactive |
| ST JAMES MOBILE HOME PARK HOME OWNERS ASSOCIATION, INC.Saint James City, FL · Inactive | Saint James City, FL | Nonprofit | Dec 2, 2016 | Inactive |
| CLEAN ENERGY INCSaint James City, FL · Inactive | Saint James City, FL | Nonprofit | Jun 7, 2017 | Inactive |
| TRIED AND TASTY - FOOD FOR LIFE, INC.Saint James City, FL · Inactive | Saint James City, FL | Nonprofit | Jun 20, 2017 | Inactive |
| CRYSTAL RIVER CORPORATION WOODSTOCK AIRSTRIPSaint James City, FL · Inactive | Saint James City, FL | Nonprofit | Nov 22, 2017 | Inactive |
| PINE ISLAND FISH, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Jan 21, 1987 | Active |
| MEDICAL SPECIAL OPERATIONS COMMUNITY, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Mar 20, 2019 | Active |
| JOHN DAVID KIRBY MINISTRIES, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Mar 10, 1987 | Active |
| PINE ISLAND BOAT CLUB, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Feb 9, 2022 | Active |
| GREATER PINE ISLAND & MATLACHA REPUBLIC CLUB CORPSt. James City, FL · Inactive | St. James City, FL | Nonprofit | Feb 11, 2022 | Inactive |
| THE STEPHEN WADE EVANGELISTIC ASSOCIATION INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Aug 4, 2022 | Active |
| PINE ISLAND STRONG INCSaint James City, FL · Inactive | Saint James City, FL | Nonprofit | Sep 30, 2022 | Inactive |
| GREATER PINE ISLAND ALLIANCE INCSt. James City, FL · Active | St. James City, FL | Nonprofit | Dec 9, 2022 | Active |
| KIWANIS CLUB OF PINE ISLAND FOUNDATION, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Apr 20, 2023 | Active |
| PINE ISLAND COVE HOMEOWNERS ASSOCIATION, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Dec 4, 1987 | Active |
| SEND A KID TO SUMMER CAMP! INCSaint James City, FL · Active | Saint James City, FL | Nonprofit | Apr 12, 2024 | Active |
| O ISLAND INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | May 24, 2024 | Inactive |
| WARRIOR FISHERMAN SOUTHWEST FLORIDA INC.St. James, FL · Active | St. James, FL | Nonprofit | Apr 14, 2025 | Active |
| MERRIMAN LEADERSHIP FOUNDATION, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Apr 8, 2025 | Active |
| PINE ISLAND GENTLEMEN'S SOCIETY INCSaint James City, FL · Active | Saint James City, FL | Nonprofit | Jun 8, 2026 | Active |
| PINE ISLAND SHORES, UNITS 10 AND 11 HOMEOWNER'S ASSOCIATION, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Apr 19, 1989 | Active |
| NORTH CHERRY ESTATES ASSOCIATION, INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Apr 27, 1989 | Inactive |
| ST. JAMES PLACE HOMEOWNERS ASSOCIATION, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Jul 3, 1989 | Active |
| CAPE CORAL DOLL GUILD, INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Nov 30, 1989 | Inactive |
| THE FRIENDS OF THE PINE ISLAND LIBRARY, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Feb 19, 1990 | Active |
| BLACK SHEEP OF PINE ISLAND CORP.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Dec 10, 1990 | Inactive |
| BUTTONWOOD KEY ASSOCIATION, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Mar 11, 1993 | Active |
| GALT ISLAND SUBDIVISION HOMEOWNERS ASSOCIATION, INC.Saint James City, FL · Active | Saint James City, FL | Nonprofit | Jun 8, 1993 | Active |
| PANTHER KEY PRESERVE, INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Mar 8, 1994 | Inactive |
| THE GREATER PINE ISLAND LIONS FOUNDATION, INC.Saint James City, FL · Inactive | Saint James City, FL | Nonprofit | May 11, 1995 | Inactive |
| PALM TREE HARBOR HOMEOWNERS ASSOCIATION, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Nov 20, 1995 | Active |
| THE LEE/DOYLE FOUNDATION, INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Apr 14, 1997 | Inactive |
| JEWISH COMMUNITY CENTER OF PINE ISLAND SOUND, INC.St. James City, FL · Inactive | St. James City, FL | Nonprofit | Jun 12, 1997 | Inactive |
| GALT ISLAND AVENUE PROPERTY RIGHTS GROUP, INC.St. James City, FL · Active | St. James City, FL | Nonprofit | Sep 22, 1997 | Active |
| BOBBY HOLLOWAY MEMORIAL, CORP.St. James City, FL · Active | St. James City, FL | Nonprofit | Jun 30, 1999 | Active |
| LARRY JONES TILE & MARBLE, INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Feb 2, 2000 | Inactive |
| KRS RESORT DEVELOPMENT, INC.St. James City, FL · Active | St. James City, FL | Corporation | Feb 10, 2000 | Active |
| KRS LAND DEVELOPMENT, INC.St. James City, FL · Active | St. James City, FL | Corporation | Feb 10, 2000 | Active |
| BRESCOTT CORPORATIONSaint James City, FL · Active | Saint James City, FL | Corporation | Mar 17, 2000 | Active |
| MY DOC NOW INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Mar 24, 2000 | Inactive |
| TROPICAL REAL ESTATE INSPECTIONS, INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Mar 27, 2000 | Inactive |
| ST. JAMES SOFT CRABS, INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Mar 31, 2000 | Inactive |
| SUNBELT #1 REALTY, INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Apr 14, 2000 | Inactive |
| WERNER FUNDING, INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Apr 17, 2000 | Inactive |
| ST. JAMES AUTOMOTIVE, INC.St. James City, FL · Active | St. James City, FL | Corporation | May 19, 2000 | Active |
| MICHAEL H. SHEVLIN, P.A.St. James City, FL · Active | St. James City, FL | Corporation | May 31, 2000 | Active |
| HIGHWAY STAR SIGN & LIGHTING SERVICES, INC.St. James City, FL · Inactive | St. James City, FL | Corporation | Aug 8, 2000 | Inactive |