| IVORY CHOICE TERMINAL CO.Jacksonville, FL · Active | Jacksonville, FL | Corporation | May 26, 2021 | Active |
| KIAMICHI RAILROAD COMPANY L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Nov 6, 2020 | Active |
| BAUXITE & NORTHERN RAILWAY COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 20, 2020 | Active |
| DALLAS, GARLAND & NORTHEASTERN RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 20, 2020 | Active |
| ARKANSAS LOUISIANA & MISSISSIPPI RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| ARKANSAS MIDLAND RAILROAD COMPANY, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| CAGY INDUSTRIES, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| MISSOURI & NORTHERN ARKANSAS RAILROAD COMPANY, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| RAIL TRANSPORTATION SOLUTIONS INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| THE PRESCOTT AND NORTHWESTERN RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| WARREN & SALINE RIVER RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| CHATTAHOOCHEE INDUSTRIAL RAILROAD INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 18, 2017 | Active |
| KWT RAILWAY, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 18, 2017 | Active |
| SOUTH CAROLINA CENTRAL RAILROAD COMPANY, LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Oct 18, 2017 | Active |
| CHATTOOGA & CHICKAMAUGA RAILWAY CO.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| COLUMBUS AND GREENVILLE RAILWAY COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| COLUMBUS & CHATTAHOOCHEE RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| COMMONWEALTH RAILWAY, INCORPORATEDJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| CONECUH VALLEY RAILWAY, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| CORPUS CHRISTI TERMINAL RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| EASTERN ALABAMA RAILWAY, LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| GEORGIA SOUTHWESTERN RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| GOLDEN ISLES TERMINAL RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| HEART OF GEORGIA RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| HILTON & ALBANY RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| LOUISIANA & DELTA RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| LUXAPALILA VALLEY RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| NORTH CAROLINA & VIRGINIA RAILROAD COMPANY, LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| POINT COMFORT & NORTHERN RAILWAY COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| ROCKDALE, SANDOW & SOUTHERN RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| SAVANNAH PORT TERMINAL RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| THREE NOTCH RAILWAY, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| WIREGRASS CENTRAL RAILWAY, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| RAILROAD MAINTENANCE SERVICES LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Apr 26, 2017 | Active |
| RAILROAD ENGINEERING SERVICES LLCJacksonville, FL · Active | Jacksonville, FL | LLC | May 1, 2015 | Active |
| FIRST COAST RAILROAD INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Apr 6, 2005 | Active |
| MID-MICHIGAN RAILROAD, INC.Location not reported, FL · Active | Location not reported, FL | Corporation | Oct 16, 2020 | Active |
| RAILAMERICA TRANSPORTATION CORP.Location not reported, FL · Inactive | Location not reported, FL | Corporation | Apr 17, 2002 | Inactive |
| YORK RAIL LOGISTICS, INC.Jacksonville, FL · Inactive | Jacksonville, FL | Corporation | Oct 17, 2017 | Inactive |
| YORK RAILWAY COMPANYJacksonville, FL · Inactive | Jacksonville, FL | Corporation | Oct 17, 2017 | Inactive |
| MARYLAND MIDLAND RAILWAY, INC.Jacksonville, FL · Inactive | Jacksonville, FL | Corporation | Oct 20, 2017 | Inactive |
| FORDYCE AND PRINCETON R.R. CO.Jacksonville, FL · Inactive | Jacksonville, FL | Corporation | Oct 16, 2020 | Inactive |
| RAILAMERICA, INC.Location not reported, FL · Inactive | Location not reported, FL | Corporation | Nov 12, 1992 | Inactive |
| RAIL LINK, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Jan 3, 1996 | Active |
| MARYLAND AND PENNSYLVANIA RAILROAD, LLCJacksonville, FL · Inactive | Jacksonville, FL | LLC | Oct 17, 2017 | Inactive |
| YORKRAIL, LLCJacksonville, FL · Inactive | Jacksonville, FL | LLC | Oct 17, 2017 | Inactive |
| ALABAMA & GULF COAST RAILWAY LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Aug 26, 1997 | Active |