| KIAMICHI RAILROAD COMPANY L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Nov 6, 2020 | Active |
| BAUXITE & NORTHERN RAILWAY COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 20, 2020 | Active |
| ARKANSAS LOUISIANA & MISSISSIPPI RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| ARKANSAS MIDLAND RAILROAD COMPANY, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| MISSOURI & NORTHERN ARKANSAS RAILROAD COMPANY, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| RAIL TRANSPORTATION SOLUTIONS INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| THE PRESCOTT AND NORTHWESTERN RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| WARREN & SALINE RIVER RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 16, 2020 | Active |
| SOUTH CAROLINA CENTRAL RAILROAD COMPANY, LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Oct 18, 2017 | Active |
| COLUMBUS AND GREENVILLE RAILWAY COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| COMMONWEALTH RAILWAY, INCORPORATEDJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| CORPUS CHRISTI TERMINAL RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| MERIDIAN & BIGBEE RAILROAD, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| NORTH CAROLINA & VIRGINIA RAILROAD COMPANY, LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| POINT COMFORT & NORTHERN RAILWAY COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| RICEBORO SOUTHERN RAILWAY, LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| ROCKDALE, SANDOW & SOUTHERN RAILROAD COMPANYJacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| SAVANNAH PORT TERMINAL RAILROAD, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Oct 17, 2017 | Active |
| THREE NOTCH RAILWAY, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| WIREGRASS CENTRAL RAILWAY, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Oct 17, 2017 | Active |
| RAILROAD MAINTENANCE SERVICES LLCJacksonville, FL · Active | Jacksonville, FL | LLC | Apr 26, 2017 | Active |
| RAILROAD ENGINEERING SERVICES LLCJacksonville, FL · Active | Jacksonville, FL | LLC | May 1, 2015 | Active |
| FIRST COAST RAILROAD INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Apr 6, 2005 | Active |
| AN RAILWAY, L.L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Aug 13, 2002 | Active |
| MID-MICHIGAN RAILROAD, INC.Location not reported, FL · Active | Location not reported, FL | Corporation | Oct 16, 2020 | Active |
| FORDYCE AND PRINCETON R.R. CO.Jacksonville, FL · Inactive | Jacksonville, FL | Corporation | Oct 16, 2020 | Inactive |
| RAIL LINK, INC.Jacksonville, FL · Active | Jacksonville, FL | Corporation | Jan 3, 1996 | Active |
| THE BAY LINE RAILROAD, L.L.C., L.C.Jacksonville, FL · Active | Jacksonville, FL | LLC | Dec 23, 1993 | Active |